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The best little site that helps you understand word usage with examples. The cell culture supernatant was regularly tested for reverse transcriptase activity. Following 5-10 min at 90 C, the hemoglobin in crude Fraction I was cooked and precipitated like mud, and the activity remained soluble in the supernatant. Depending on the filter design, flow rates may have to be adjusted accordingly or the layer of supernatant water on the filter will get too high.

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